4M85
Crystal structure of N-acetyltransferase from Staphylococcus aureus Mu50
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.460, 68.970, 73.460 |
| Unit cell angles | 67.23, 91.69, 75.27 |
Refinement procedure
| Resolution | 36.280 - 2.000 |
| R-factor | 0.19409 |
| Rwork | 0.192 |
| R-free | 0.23912 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u6m |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.246 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.280 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.083 |
| Number of reflections | 49478 |
| Completeness [%] | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 22%PEG8000,50mM Potassium phosphate monobasic, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
