4M7K

Crystal structure of anti-tissue factor antibody 10H10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2007-09-11
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	81.770, 135.510, 88.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.2058
Rwork	0.205
R-free	0.24276
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1VGE 1mvu AND 1D5I
RMSD bond length	0.007
RMSD bond angle	1.059
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.060	0.365
Number of reflections	38664
<I/σ(I)>	26.3	4.2
Completeness [%]	99.4	95.3
Redundancy	9.5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M cacodylate, pH 6.5, 24% PEG8000, 1 M sodium acetate, 5% PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 293K