4M7E

Structural insight into BL-induced activation of the BRI1-BAK1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	298
Detector technology	CCD
Collection date	2013-06-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 31
Unit cell lengths	145.911, 145.911, 166.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.904 - 3.602
R-factor	0.2226
Rwork	0.220
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rgz
RMSD bond length	0.014
RMSD bond angle	2.091
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.600
Number of reflections	45084
Completeness [%]	98.3
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4	298	0.1M Citric Acid, 2.0M (NH4)2SO4, pH 4.0, EVAPORATION, temperature 298.0K