4M70
Crystal structure of potato Rx-CC domain in complex with RanGAP2-WPP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.085, 91.142, 87.762 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 28.648 - 2.103 |
| R-factor | 0.2541 |
| Rwork | 0.253 |
| R-free | 0.27560 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.943 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.648 | 2.180 |
| High resolution limit [Å] | 2.103 | 2.100 |
| Rmerge | 0.053 | |
| Number of reflections | 65379 | |
| Completeness [%] | 100.0 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 10mM Tis, 100 mM NaCl, 39%(V/V) Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
