4M6S

Crystal structure of the R111K:R132Y:Y134F:T54V:R59W:A32W mutant of the Cellular Retinoic Acid Binding Protein Type II in complex with All-Trans Retinal at 2.38 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0782
Spacegroup name	P 31 2 1
Unit cell lengths	56.629, 56.629, 111.318
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.880 - 2.470
R-factor	0.2058
Rwork	0.204
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g7b
RMSD bond length	0.008
RMSD bond angle	1.128
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.880
High resolution limit [Å]	2.380	2.380
Number of reflections	8713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.1 M malonate, pH 6.0, 12% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K