4M6F

Dimer of the G-Segment Invertase bound to a DNA substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0849
Spacegroup name	P 64 2 2
Unit cell lengths	119.800, 119.800, 343.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 4.990
R-factor	0.23
Rwork	0.229
R-free	0.28800
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	5.250
High resolution limit [Å]	4.990	4.970
Rmerge	0.111
Completeness [%]	94.5	94.6
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	3-5% Ammonium Sulfate 20% ethylene glycol 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	3-5% Ammonium Sulfate 20% ethylene glycol 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	3-5% Ammonium Sulfate 20% ethylene glycol 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K