4M6E
The high resolution structure of tyrocidine A reveals an amphipathic dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.8984 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 33.390, 33.390, 50.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.000 - 0.950 |
| R-factor | 0.1347 |
| Rwork | 0.134 |
| R-free | 0.14220 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.787 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SnB |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.000 |
| High resolution limit [Å] | 0.950 |
| Rmerge | 0.040 |
| Number of reflections | 6973 |
| <I/σ(I)> | 48 |
| Completeness [%] | 98.8 |
| Redundancy | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | tyrocidine in methanol at 30 mg/mL, reservoir methanol:MPD (1:5 v/v), VAPOR DIFFUSION, SITTING DROP, temperature 291K |
