4M6D

Crystal structure of the aptamer minF-lysozyme complex.

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 1
Unit cell lengths	43.914, 132.289, 131.539
Unit cell angles	118.59, 96.39, 96.27

Refinement procedure

Resolution	38.040 - 2.680
R-factor	0.16832
Rwork	0.165
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m4o
RMSD bond length	0.013
RMSD bond angle	1.421
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.1)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.680	7.320	2.700
Rmerge	0.067	0.037	0.629
Number of reflections	67209
<I/σ(I)>	12.1
Completeness [%]	94.9	90.6	90.9
Redundancy	1.9	1.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	1.1M Malonic Acid, 0.15 M Ammonium Citrate Tribasic, 0.072 M Succinic Acid, 0.18 M DL-Malic Acid, 0.24 M Sodium Acetate, 0.3 M Sodium Formate, 0.096 M Ammonium Tartrate Dibasic, Final pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K