4M5P
OYE2.6 Y78W, I113C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 126.487, 126.487, 123.307 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.040 - 1.503 |
| R-factor | 0.1438 |
| Rwork | 0.141 |
| R-free | 0.16970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tjl |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.086 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.040 | |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.046 | |
| Number of reflections | 111288 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 99.9 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 2.4M Sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
