4M5B

Crystal Structure of an Truncated Transition Metal Transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	64.371, 103.160, 120.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.410 - 1.833
R-factor	0.1842
Rwork	0.183
R-free	0.20150
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.023
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.830	1.830
Number of reflections	34896
Completeness [%]	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	291.15	35% PEG 300, 0.05M glycine, 0.1M sodium chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K