4M57

Crystal structure of the pentatricopeptide repeat protein PPR10 from maize

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-05
Detector	RAYONIX MX325HE
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	68.373, 176.536, 64.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.080 - 2.860
R-factor	0.24
Rwork	0.240
R-free	0.25400
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.318
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.950
High resolution limit [Å]	2.850	2.850
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1.8M sodium formate, 0.1M Bis-Tris propane, 0.04M DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K