4M4O
Crystal structure of the aptamer minE-lysozyme complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.216, 30.609, 89.817 |
| Unit cell angles | 90.00, 122.45, 90.00 |
Refinement procedure
| Resolution | 37.980 - 2.000 |
| R-factor | 0.1822 |
| Rwork | 0.181 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jkb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.162 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.056 | 0.027 | 0.348 |
| Number of reflections | 22172 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 98.8 | 95.4 | 90.3 |
| Redundancy | 3.1 | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.17M Sodium Acetate, 0.085M Tris-HCl, pH 8.5, 25.5% PEG-4000, 15% v/v glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
