4M4E

TRAF domain of human TRAF4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	57.295, 88.205, 118.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.031 - 2.600
R-factor	0.2079
Rwork	0.200
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ca4
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	18582
Completeness [%]	96.5	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	289	0.2M Ammonium fluoride, 20%(w/v) Polyethylene glycol 3350, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K