4M46
Crystal structure of a green-emitter native of Lampyris turkestanicus luciferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 41 |
| Unit cell lengths | 85.100, 85.100, 96.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.630 - 2.700 |
| R-factor | 0.21232 |
| Rwork | 0.210 |
| R-free | 0.24761 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lci |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.825 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 96.630 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.167 |
| Number of reflections | 18984 |
| Completeness [%] | 88.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 100 mM MES Sodium salt, 40% v/v PEG 400, 5% w/v PEG 3000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
