4M44
Crystal structure of hemagglutinin of influenza virus B/Yamanashi/166/1998 in complex with avian-like receptor LSTa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2012-06-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.130, 101.520, 137.370 |
| Unit cell angles | 90.00, 115.22, 90.00 |
Refinement procedure
| Resolution | 39.364 - 2.500 |
| R-factor | 0.1874 |
| Rwork | 0.186 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.204 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: dev_1391)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.370 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.091 | 0.494 |
| Number of reflections | 75849 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 23% PEG2000MME, 0.1 M Tris, 0.2 M TMNO, pH 8.5, VAPOR DIFFUSION, HANGING DROP |
