4M3W

RB69 DNA polymerase ternary complex with dT/dG at position n-4 of primer/template duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	110
Detector technology	CCD
Collection date	2012-10-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	78.518, 118.774, 129.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.410 - 2.100
R-factor	0.17978
Rwork	0.178
R-free	0.22037
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.053
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	58582
Completeness [%]	82.6	93.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	150 mM CaCl2, 12% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5) , VAPOR DIFFUSION, temperature 298K