4M3L

Crystal Structure of the coiled coil domain of MuRF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	70.790, 24.410, 75.390
Unit cell angles	90.00, 107.65, 90.00

Refinement procedure

Resolution	19.815 - 2.100
R-factor	0.2145
Rwork	0.212
R-free	0.26150
Structure solution method	ARCIMBOLDO (fragment based ab initio)
RMSD bond length	0.006
RMSD bond angle	0.836
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Arcimboldo
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	14624
<I/σ(I)>	17.24	3.31
Completeness [%]	97.7	99.3
Redundancy	3.64	3.74

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293.15	35% MPD, 0.1M sodium acetate, 20% glycerol, 20mM sodium fluoride, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K