4M34
Crystal structure of gated-pore mutant D138H of second DNA-Binding protein under starvation from Mycobacterium smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-07-23 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | H 3 2 |
Unit cell lengths | 89.490, 89.490, 420.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.050 |
R-factor | 0.16871 |
Rwork | 0.167 |
R-free | 0.20654 |
Structure solution method | Direct refinement against model PDB |
Starting model (for MR) | 2z90 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.838 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.140 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.042 | 0.101 |
Number of reflections | 41333 | |
<I/σ(I)> | 37 | 18.5 |
Redundancy | 8.8 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil | 6.5 | 298 | 100mM MgCl2, 0.1M sodium cacodylate, 20% PEG3350, pH 6.5, Microbatch under oil, temperature 298K |