4M2P
Crystal structure of a non-myristoylated C39D recoverin mutant with one calcium ion bound to EF-hand 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-15 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | I 4 |
Unit cell lengths | 84.220, 84.220, 59.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.800 - 1.450 |
R-factor | 0.205 |
Rwork | 0.204 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omr |
RMSD bond length | 0.006 |
RMSD bond angle | 1.108 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 1.530 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.095 | 0.421 |
Number of reflections | 36942 | |
<I/σ(I)> | 9.6 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.1 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 2.4 M SODIUM MALONATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |