4M2O
Crystal structure of a non-myristoylated C39A recoverin mutant with one calcium ion bound to EF-hand 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 4 |
| Unit cell lengths | 84.050, 84.050, 58.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.700 - 1.500 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.700 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.088 | 0.386 |
| Number of reflections | 32871 | |
| <I/σ(I)> | 10.1 | 2.9 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 5.3 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 296 | 2.0 M AMMONIUM CITRATE, pH 7.0, temperature 296K |
