4M2G
Crystal structure of non-heme iron oxygenase OrfP in complex with Fe, succinate, and (3R,4R)-dihydroxy-L-Arg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-05-13 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.287, 116.211, 96.163 |
Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
Resolution | 26.150 - 2.390 |
R-factor | 0.21656 |
Rwork | 0.214 |
R-free | 0.27074 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wbo |
RMSD bond length | 0.006 |
RMSD bond angle | 1.060 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.480 |
High resolution limit [Å] | 2.390 | 2.390 |
Number of reflections | 55626 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 100mM Bis Tris Propane, 200mM NaF, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 293K |