4M2C
Crystal structure of non-heme iron oxygenase OrfP in complex with Fe and D-Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-03-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.034, 116.369, 95.800 |
| Unit cell angles | 90.00, 91.44, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.22594 |
| Rwork | 0.223 |
| R-free | 0.27571 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m23 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 57805 | |
| Completeness [%] | 98.4 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 100mM Bis Tris Propane, 200mM NaF, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 293K |
