4M24
Crystal structure of the endo-1,4-glucanase, RBcel1, in complex with cellobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979718 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.700, 63.250, 98.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.112 - 1.386 |
R-factor | 0.1263 |
Rwork | 0.124 |
R-free | 0.14890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gzj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.177 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER ((phenix.automr: 1.8.2_1309)) |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.260 | 1.440 |
High resolution limit [Å] | 1.386 | 1.390 |
Rmerge | 0.068 | 0.690 |
Number of reflections | 64593 | |
<I/σ(I)> | 30.3 | 4.3 |
Completeness [%] | 97.6 | 93.3 |
Redundancy | 13.8 | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | RBCel1 in 20 mM NaPi pH 6.5 and cellobiose 1% , was mixed 2:2 with well buffer (100mM Tris HCl pH 6.0 with 20 % w/v polyethylene glycol 600) using the hanging drop method with 500mL well buffer in the well of the crystallization tray, VAPOR DIFFUSION, HANGING DROP, temperature 292K |