4M1Z
Crystal structure of MycP1 with the N-terminal propeptide removed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97906 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.311, 83.611, 98.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.280 - 2.250 |
| R-factor | 0.1688 |
| Rwork | 0.166 |
| R-free | 0.22183 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kb5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.361 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.102 | 0.444 |
| Number of reflections | 33595 | |
| <I/σ(I)> | 12.912 | 2.845 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 283 | 0.1M sodium citrate, 20% (v/v) 2-propanol, 20% (w/v) PEG 4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
