4M1R
Structure of a novel cellulase 5 from a sugarcane soil metagenomic library
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.458 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.888, 87.512, 66.630 |
| Unit cell angles | 90.00, 98.55, 90.00 |
Refinement procedure
| Resolution | 28.550 - 1.800 |
| R-factor | 0.13284 |
| Rwork | 0.131 |
| R-free | 0.17271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.876 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 41174 |
| Completeness [%] | 93.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 35% (v/v) isopropanol, 30% (w/v) PEG3350 and 100 mM Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
