4M1Q
Crystal structure of L-lactate dehydrogenase from Bacillus selenitireducens MLS10, NYSGRC Target 029814.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.878, 81.878, 210.268 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.390 - 1.600 |
| R-factor | 0.1812 |
| Rwork | 0.180 |
| R-free | 0.19600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.311 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.104 | 0.061 | 0.937 |
| Number of reflections | 108623 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 99.9 | 99.5 | 100 |
| Redundancy | 6.8 | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | 0.1 M Na2HPO4/KH2PO4, pH 6.2, 35% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
