4M1L
Complex of IQCG and Ca2+-bound CaM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 83 |
| Detector technology | CCD |
| Collection date | 2011-10-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.491, 40.491, 179.689 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.642 - 2.100 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ewt |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.654 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.143 | |
| Number of reflections | 10570 | |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 97.6 | 94.2 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.6M ammonium sulfate, 1xM lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
