4M1K
Crystal structure of elongation factor G (EFG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.989, 86.767, 123.118 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.995 - 2.952 |
| R-factor | 0.1985 |
| Rwork | 0.194 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fnm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.227 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.950 |
| Rmerge | 0.549 | |
| Number of reflections | 17685 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 5mM Tris-HCl (pH 7.6), 10mM MgCl2, 5mM GDP, 20-23% PEG8000, 0.1M Tris-Cl PH7.5-7.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
