4M0D

Crystal structure of MurQ from H.influenzae in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	65
Detector technology	IMAGE PLATE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	76.127, 111.650, 134.509
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.563 - 2.577
R-factor	0.1888
Rwork	0.183
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nri
RMSD bond length	0.008
RMSD bond angle	1.267
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.560
High resolution limit [Å]	2.580	2.580
Number of reflections	34265
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.2 M Nacl, 0.1 M Bis-Tris:HCl, pH 5.5, 25% (w/v) PEG-3350, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K