4M03

C-terminal fragment(residues 576-751) of binding region of SraP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.07138
Spacegroup name	P 31 2 1
Unit cell lengths	34.756, 34.756, 237.725
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.100 - 2.240
R-factor	0.28155
Rwork	0.279
R-free	0.32630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m02
RMSD bond length	0.012
RMSD bond angle	1.569
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.240	2.240
Rmerge	0.131	0.389
Number of reflections	8890
<I/σ(I)>	16.732	8.775
Completeness [%]	99.6	96.7
Redundancy	8.6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	1.5M NaKPO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K