4M00
Crystal structure of the ligand binding region of staphylococcal adhesion SraP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.018, 118.018, 136.407 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.540 - 2.050 |
| R-factor | 0.18193 |
| Rwork | 0.181 |
| R-free | 0.20606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m01 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.350 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.137 | 0.385 |
| Number of reflections | 69226 | |
| <I/σ(I)> | 13.8 | 5.754 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 301 | 0.8M ammonium sulfate, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
