4LZT

ATOMIC RESOLUTION REFINEMENT OF TRICLINIC HEW LYSOZYME AT 295K

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1996-12
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	27.240, 31.870, 34.230
Unit cell angles	88.52, 108.53, 111.89

Refinement procedure

Resolution	21.000 - 0.950
R-factor	0.1144
R-free	0.14700
Structure solution method	STRUCTURE KNOWN
Starting model (for MR)	2lzt
RMSD bond length	0.016
RMSD bond angle	0.033
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-96
Refinement software	SHELXL-96

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.000	0.970
High resolution limit [Å]	0.950	0.950
Rmerge	0.060	0.273
Total number of observations	289540 *
Number of reflections	61265
<I/σ(I)>	14.4	4.7
Completeness [%]	96.5	94.9
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.5	296 *	BATCH METHOD USED. 1% PROTEIN SOLUTION IN 100MM SODIUM ACETATE PH 4.5-4.6. SODIUM NITRATE ADDED TO A CONCENTRATION OF 20MGS/ML. CRYSTALS GROWN AT ROOM TEMPERATURE., batch method

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein solution	1 (%)
2	1	1	sodium acetate	100 (mM)
3	1	2	sodium nitrate	2 (%(w/v))