4LYR
Glycoside Hydrolase Family 5 Mannosidase from Rhizomucor miehei, E301A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.984, 76.994, 55.084 |
| Unit cell angles | 90.00, 113.34, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.13478 |
| Rwork | 0.132 |
| R-free | 0.18044 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.860 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.990 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.041 | |
| Number of reflections | 16116 | |
| Completeness [%] | 91.5 | 55.3 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 16% PEG 4000, 0.1M HEPES buffer pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
