4LYL
Crystal structure of uracil-DNA glycosylase from cod (Gadus morhua) in complex with the proteinaceous inhibitor UGI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2009-12-10 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.210, 86.920, 175.370 |
| Unit cell angles | 90.00, 90.35, 90.00 |
Refinement procedure
| Resolution | 29.480 - 1.930 |
| R-factor | 0.2393 |
| Rwork | 0.237 |
| R-free | 0.28340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 175.000 | 2.040 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.073 | 0.413 |
| Number of reflections | 199006 | |
| <I/σ(I)> | 10.76 | 3.29 |
| Completeness [%] | 91.2 | 86.6 |
| Redundancy | 2.94 | 3.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 17% PEG 4000, 4% PEG 550 MME, 0.27M LITHIUM SULFATE, 0.01M SODIUM BROMIDE, 0.1M TRIS-HCL, PH 7.4, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277.0K |
