4LYA
EssC (ATPases 2 and 3) from Geobacillus thermodenitrificans (SeMet)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.346, 62.971, 83.888 |
| Unit cell angles | 90.00, 107.47, 90.00 |
Refinement procedure
| Resolution | 49.930 - 2.450 |
| Rwork | 0.208 |
| R-free | 0.26340 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 18846 | |
| Completeness [%] | 97.4 | 93.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 30% PEG300, 0.1M Na Acetate, pH 4.6, 20% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
