4LY0
Crystal structure WlaRD, a sugar 3N-formyl transferase in the presence of dTDP-Glc and 10-N-Formyl-THF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-01 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.641, 65.286, 135.805 |
| Unit cell angles | 90.00, 111.73, 90.00 |
Refinement procedure
| Resolution | 45.720 - 1.600 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lxq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.240 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.600 | 1.900 |
| Rmerge | 0.046 | 0.305 |
| Number of reflections | 96212 | |
| <I/σ(I)> | 15.6 | 2.2 |
| Completeness [%] | 92.8 | 79.1 |
| Redundancy | 3.3 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 11-15% PEG 8000, 200 mM NaCl, 5 mM N5-formyl-THF, 5 mM TDP, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
