4LXX
Crystal structure WlaRD, a sugar 3N-formyl transferase in the presence of dTDP-Fuc3NFo and 5-N-Formyl-THF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.992, 64.825, 134.798 |
| Unit cell angles | 90.00, 110.60, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.450 |
| R-factor | 0.1827 |
| Rwork | 0.181 |
| R-free | 0.21180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lxq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.710 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.071 | 0.120 |
| Number of reflections | 130605 | |
| <I/σ(I)> | 56.4 | 11.5 |
| Completeness [%] | 94.5 | 85.5 |
| Redundancy | 4.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 11-15% PEG 8000, 200 mM NaCl, 5 mM N-5-formyl-THF, 5 mM TDP-Fuc3NFo, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
