4LXT
Crystal structure WlaRD, a sugar 3N-formyl transferase in the presence of dTdp-Qui3N and 5-N-Formyl-THF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-12 |
Detector | ADSC QUANTUM 210r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.775, 65.148, 134.718 |
Unit cell angles | 90.00, 110.51, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.400 |
R-factor | 0.179 |
Rwork | 0.177 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lxq |
RMSD bond length | 0.015 |
RMSD bond angle | 2.071 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.045 | 0.080 |
Number of reflections | 143095 | |
<I/σ(I)> | 48.3 | 15.5 |
Completeness [%] | 92.9 | 84.9 |
Redundancy | 3.8 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 295 | 11-15% PEG 8000, 200 mM NaCl, 5 mM N-5formyl-THF, 5 mM TDP-Qui3N, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |