4LXC
The antimicrobial peptidase lysostaphin from Staphylococcus simulans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 282.013, 282.013, 282.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.670 - 3.500 |
| R-factor | 0.26873 |
| Rwork | 0.268 |
| R-free | 0.28684 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1R77 2b0p |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.295 | 0.908 |
| Number of reflections | 48693 | |
| <I/σ(I)> | 17.34 | 5.34 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 36.4 | 37.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M MES and 1.6 M magnesium sulphate, 2 mM tetraglycine phosphinic acid. EDTA and ammonium sulphate as additives improved crystal quality, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
