4LX2

Crystal structure of Myo5a globular tail domain in complex with melanophilin GTBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.980110
Spacegroup name	C 1 2 1
Unit cell lengths	99.870, 41.110, 109.580
Unit cell angles	90.00, 115.63, 90.00

Refinement procedure

Resolution	35.572 - 1.500
R-factor	0.1659
Rwork	0.164
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lx0
RMSD bond length	0.011
RMSD bond angle	1.279
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	1.590
High resolution limit [Å]	1.500	1.500
Number of reflections	64638
Completeness [%]	99.1	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG MPD, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K