4LWS
EsxA : EsxB (SeMet) hetero-dimer from Thermomonospora curvata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2013-03-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9797 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.253, 69.805, 128.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.660 - 2.000 |
| Rwork | 0.190 |
| R-free | 0.23840 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.183 | 1.818 |
| High resolution limit [Å] | 1.775 | 1.755 |
| Number of reflections | 47222 | |
| Completeness [%] | 96.7 | 75.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 70mM sodium acetate pH 4.6 5.6% (w/v) PEG 4000 30% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
