4LVN
Crystal structure of PfSUB1-prodomain-NIMP.M7 Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-15 |
| Detector | Pilatus 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.500, 74.790, 78.850 |
| Unit cell angles | 90.00, 103.33, 90.00 |
Refinement procedure
| Resolution | 29.882 - 2.250 |
| R-factor | 0.1975 |
| Rwork | 0.195 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1BH6 AND 1MLC |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.938 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (AutoMR) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.520 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.108 | 0.409 |
| Number of reflections | 38990 | |
| <I/σ(I)> | 7.95 | 2.67 |
| Completeness [%] | 98.3 | 99.59 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 20% PEG 3350, 0.2M ammonium formate, 150mM NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
