4LVK
MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt+3'Phosphate). Mn-bound crystal structure at pH 4.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97300 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 112.743, 112.743, 91.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.930 - 2.370 |
| R-factor | 0.20312 |
| Rwork | 0.201 |
| R-free | 0.24987 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.864 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.510 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.107 | |
| Number of reflections | 13543 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.3 | 97.8 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION + seeding | 4.6 | 293 | 22% PEG 4000, 0.2M Sodium Chloride, 0.1M Sodium Acetate pH4.6., VAPOR DIFFUSION + seeding, temperature 293K |
