4LUY
Crystal structure of CdALR mutant K 271 T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-02 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.970, 154.330, 55.770 |
| Unit cell angles | 90.00, 113.63, 90.00 |
Refinement procedure
| Resolution | 28.830 - 2.600 |
| R-factor | 0.18498 |
| Rwork | 0.181 |
| R-free | 0.25199 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lus |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.577 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.590 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.097 | 0.158 |
| Number of reflections | 21924 | |
| <I/σ(I)> | 4.8 | 1.9 |
| Completeness [%] | 97.0 | 55.7 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.17M LiSO4, 0.085M Tris-HCL pH 8.5, 25.5% PEG4000, 15.5% Glycerol, 0.02% betamercaptoethanol, 10 mM PLP , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
