4LUP
Crystal structure of the complex formed by region of E. coli sigmaE bound to its -10 element non template strand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-30 |
| Detector | PSI PILATUS 2M-F |
| Wavelength(s) | 0.80000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.050, 36.480, 73.370 |
| Unit cell angles | 90.00, 92.48, 90.00 |
Refinement procedure
| Resolution | 35.075 - 1.200 |
| R-factor | 0.1474 |
| Rwork | 0.147 |
| R-free | 0.16480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2map |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.994 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.075 | 35.075 | 1.300 |
| High resolution limit [Å] | 1.200 | 30.000 | 1.200 |
| Rmerge | 0.043 | 0.016 | 0.657 |
| Number of reflections | 64851 | ||
| <I/σ(I)> | 28.6 | 36.46 | 4.38 |
| Completeness [%] | 100.0 | 50 | 100 |
| Redundancy | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | The complex crystallized spontaneously at a concentration of 0.5 mM in the NMR buffer (10 mM Na-Phosphate buffer pH 6.5, 50 mM NaCl), BATCH, temperature 293K |
