4LTU
Crystal Structure of Ferredoxin from Rhodopseudomonas palustris HaA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-17 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.983, 73.145, 78.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.310 |
| R-factor | 0.23161 |
| Rwork | 0.228 |
| R-free | 0.29775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r7s |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.598 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.620 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.141 | 0.412 |
| Number of reflections | 8261 | |
| <I/σ(I)> | 7.75 | 2.42 |
| Completeness [%] | 98.4 | 95 |
| Redundancy | 4.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.01M Cobalt chloride hexahydrate, 0.1M MES pH 6.0, 1.8M Ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
