4LTQ
Bacterial sodium channel in low calcium, P42 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 42 |
| Unit cell lengths | 181.136, 181.136, 94.021 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 5.500 |
| R-factor | 0.26759 |
| Rwork | 0.266 |
| R-free | 0.29412 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lto |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.216 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 5.800 |
| High resolution limit [Å] | 5.500 | 5.500 |
| Rmerge | 0.059 | 1.916 |
| Number of reflections | 9811 | |
| <I/σ(I)> | 9.8 | 0.7 |
| Completeness [%] | 97.3 | 86.1 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 200 mM magnesium chloride, 30% PEG400, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
