4LTP
Bacterial sodium channel in high calcium, I222 space group, crystal 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 147.265, 161.499, 161.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.800 |
| R-factor | 0.22708 |
| Rwork | 0.225 |
| R-free | 0.27289 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lto |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.624 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.010 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.181 | 3.306 |
| Number of reflections | 19162 | |
| <I/σ(I)> | 6 | 0.5 |
| Completeness [%] | 99.4 | 97 |
| Redundancy | 8.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 200 mM calcium chloride, 30% PEG400, 100 mM sodium acetate, pH 5.0, 0.5 M TMAO, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
