4LT8

Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-G on the Ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-28
Detector	ADSC Q315
Wavelength(s)	0.97924
Spacegroup name	P 21 21 21
Unit cell lengths	209.213, 448.453, 619.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	189.596 - 3.140
R-factor	0.231426783863
Rwork	0.230
R-free	0.26235
RMSD bond length	0.006
RMSD bond angle	1.099
Data scaling software	XSCALE
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			3.170
High resolution limit [Å]	3.090	13.820	3.090
Rmerge	0.274	0.012	0.012
Number of reflections	1047220	12045	74306
<I/σ(I)>	6.74	23.38	1.08
Completeness [%]	99.5	96	95.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K