4LT2
HEWL co-crystallized with Carboplatin in non-NaCl conditions: crystal 2 processed using the EVAL software package
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 127 |
Detector technology | CCD |
Collection date | 2013-05-08 |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.294, 77.294, 36.628 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.150 - 2.000 |
R-factor | 0.21041 |
Rwork | 0.208 |
R-free | 0.26507 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w1y |
RMSD bond length | 0.016 |
RMSD bond angle | 1.815 |
Data reduction software | EVAL15 |
Data scaling software | EVAL15 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.150 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.193 | 1.410 |
Number of reflections | 7501 | |
<I/σ(I)> | 14.2 | 1.1 |
Completeness [%] | 94.9 | 93.7 |
Redundancy | 21.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 294 | 2 uL protein/carboplatin/DMSO solution [20 mg (0.6 mM) HEWL + 1.4 mg (1.8 mM) carboplatin in 1 mL distilled water + 75 uL DMSO] + 2 uL reservoir solution [65% MPD, 0.1 M citric acid, pH 4.0] against 1 mL of reservoir solution, crystals grew after ~4 weeks, data collected 5 weeks after crystal growth, VAPOR DIFFUSION, HANGING DROP, temperature 294K |